We describe the design of micropatterned surfaces for single cell studies. based on thermoresponsive polymer brushes. We show that brushes made of poly(N-isopropylacrylamide) grafted at high surface density display excellent protein and cell anti-adhesive properties. Such brushes are readily patterned at the micron scale via deep UV photolithography. https://atlanticknifers.shop/product-category/key-module/